Re: I'm back too and PCR testing

From: Dr. Ainsworth (ainsron@sbcglobal.net)
Tue Dec 23 08:06:38 2003


The HerpeSelect-1 (ELISA) is 96% sensitive and 97% specific. The HerpeSelect-2 is 99% sensitive and 98% specific. That is in comparison to the Western blot test. They take 3 wks to seroconvert, compared to a delay in the western blot of 6 wks to 3 months. Seroconversion, of course, can be useful in distinguishing a primary from a recurrent lesion. Although cultures are the preferred method to detect virus, the problem with viral culture is that they have up to 50% false negative rates because the virus is only shed from a recurrent lesion for about 2-3 days and require a week to read. Modified culture techniques (ELVIS) provide more rapid turnaround, but don't separate HSV-1 from HSV-2. [Clinical challenges in primary care. AMA. 2002.]

>Interesting approach. Do you know if there is much cross reactivity
>between HSV 1 and 2 and the pcr primers?
>
>On Monday, December 22, 2003, at 11:42 AM, ginny lee, CNM wrote:
>
>> agreed about the wet mount, we still do in office. I have used the PCR
>> primarily for the recurrent patients as well
>> works nicely for those patients who insist something is still "wrong"
>>
>> I do serology for all the potential hsv cases as well
>> have had an increasing # of cases come back for hsv type 1 on culture
>>
>> ginny
>>> --
>>> **********************************************************************
>>> **
>>> **********************************************************************

>>> Charlie Chambers
>>> Hood River, OR
>>> cchamber@alumni.rice.edu
>>>
>>> "No matter where you go...
>>> there you are."
>>> Dr. Buckaroo Banzai
>>> **********************************************************************
>>> **
>>> **********************************************************************

>>>
>>> **********************************************************************
>>> **
>>> **********************************************************************

>>>
>************************************************************************
>******
>

>--
>************************************************************************
>Charlie Chambers
>Hood River, OR USA

>cchamber@alumni.rice.edu
>
>"All good things...come by grace,
>and grace comes by art,
>and art does not come easy."
>
>Norman Maclean
>************************************************************************
>*******
>************************************************************************

>





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