rH D and trofoblast
From: Antonio Valdez (avaldez@juarez.chih.cablemas.com)
Wed Aug 2 12:57:35 2000
Acording to this...there is no rH D on trofoblast, so I insist...why the
rhogam?
Thank for your answers
Antonio Valdez-Torres MD FACOG
Ciudad Juarez, Chihuahua MEXICO
>----- Original Message -----
From: Carlos Brito <clbmtoc@telcel.net.ve>
To: Multiple recipients of list OBGIN-L <obgin-l@mail.medispecialty.com>
Sent: Tuesday, August 01, 2000 7:29 PM
Subject: Re: Rhogam and molar pregnancy
> Clinical utility of fetal RhD typing in alloimmunized pregnancies by
> means of polymerase chain reaction on amniocytes or chorionic villi
>
> Nicholas M. Fisk, PhDa [MEDLINE LOOKUP]
> Phillip Bennett, PhDa [MEDLINE LOOKUP]
> Ruth M. Warwick, MBb [MEDLINE LOOKUP]
> Elizabeth A. Letsky, MBa [MEDLINE LOOKUP]
> Ross Welch, MDc [MEDLINE LOOKUP]
> Janet I. Vaughan, MBa [MEDLINE LOOKUP]
> Gudrun Moore, PhDa [MEDLINE LOOKUP]
> London and Liverpool, United Kingdom
>
> Abstract TOP
>
> Espero esto te ayude, exito
>
> February 1998 . Volume 178 . Number 2
>
> Absence of expression of RhD by human trophoblast cells
>
> Alexandra Benachi, MDa [MEDLINE LOOKUP]
> Henk S. P. Garritsen, MD, PhDb [MEDLINE LOOKUP]
> Catherine M. Howard, PhDa [MEDLINE LOOKUP]
> Phillip Bennett, MD, PhDa [MEDLINE LOOKUP]
> Nicholas M. Fisk, MB, PhDa [MEDLINE LOOKUP]
>
> London, United Kingdom, and Münster, Germany
>
> Abstract TOP
>
> OBJECTIVE: Our purpose was to determine whether the RhD gene is
> expressed in trophoblast at any stage of gestation.
> STUDY DESIGN: Trophoblast and fetal tissue were obtained from 18
> pregnancies at 8 to 40 weeks' gestation. Deoxyribonucleic acid and
> ribonucleic acid were extracted from trophoblast. Complementary
> deoxyribonucleic acid was synthesized from ribonucleic acid, and reverse
> transcriptase-polymerase chain reaction was performed using primers
> specific for the RhD gene. Deoxyribonucleic acid was extracted from
> fetaltissue to determine the fetal RhD status by means of polymerase
> chain reaction. Antigen expression was also sought by analytic
> cytometric analysis (flow cytometry and immunocytochemistry) using a
> monoclonal anti-D antibody.
> RESULTS: Trophoblast was studied from various combinations of
> RhD-positive and RhD-negative fetuses (on deoxyribonucleic acid) from
> mothers to find no RhD gene expression in any sample. Flow cytometry
> and immunocytochemistry confirmed this by demonstrating no RhD antigen
> sites on trophoblast cells.
> CONCLUSION: Contrary to a previous report, we conclude that the RhD gene
> is not expressed in human trophoblast in any trimester. (Am J Obstet
> Gynecol 1998;178:294-9.)
>
> At Tue, 1 Aug 2000, Antonio Valdez wrote:
> >
> >> Estimados colegas, existe una publicacion de Clinicas de Ginecologia y
> >> obstetricia de norteamerica de aprox .1986 en donde presentan trabajos
> >> que demuestran la necesidad del uso de Rhogam, alli entre otras cosas
> >> tambien se permiten mas tiempo para aplicar el rhogam ( disculpa lo
> >> inexacto de la fecha, pero no tengo a la mano la publicacion..Exito
> >> desde venezuela.At Sun, 30 Jul 2000, Pablo Garcia wrote:
> >
> > Estimados amigos:
> > Reciban un cordial saludo y una disculpa por haber enviado mi mensaje
en
> >ingles (iba a otra lista) y no en nuestro querido castellano...
> >Pablo: NO encuentro dicha referencia, y sigo en duda porquer hay que
aplicar
> >rhogam en una paciente rh negativo a quien le evacuas un embarazo molar
> >total si nunca hubo feto NI sangre fetal que pudiera entrar en la
> >circulacion materna... Voy a seguir buscando, y ojala alguien nos ayude a
> >salir de dudas
> >
> >--
> >Antonio Valdez Torres
> >Ciudad Juarez, Chihauhua MEXICO
> >
>
